BIOL 2420 Gram Staining Biology Paper

BIOL 2420 Gram Staining Biology Paper

Instructions/Descriptions

BIOL 2420 Gram Staining Biology Paper

BIOL 2420, Gram, Staining, Biology, Paper

Unknown number: ____________ (to be inserted when you know it)

Neisseria polysaccharea (to be substituted here when you know it)

Introduction 03-01-21

(Approximately 2-3 complete sentences in third person) (date 1st submitted)

What is this project about?

What applications and techniques will be used (including relevant information/talents students already possess both those acquired during the part of this course already covered as well as general life skills)?

Plan 03-01-21

(Approximately 1 – 2 paragraphs in third person) (Due first week of project)

How is any bacterial culture characterized (i.e., growth characteristics of broth, plate, slant)? Why?

How is a pure culture established (i.e., broth to plate to slants to Gram stains of both slants that match Gram stain of original broth)? Why?

How would anyone with an unknown bacterium select the correct strike chart? (Use Gram stain charts to determine tests needed to lead to appropriate strike chart.) If GN? If GPC? If GPR? Explain all three.

Evidence 03-10-21?

(Approximately 1 paragraph) (Due when strike chart selected)

How did you select the correct strike chart? (Use Gram stain charts to determine tests needed to lead to appropriate strike chart. Include only those results needed to determine strike chart. Mention chart by name.)

Analysis 05-03-21

(Approximately 1 – 2 paragraphs) (Due when final paper is due)

Discuss how the Descriptive Chart is used to collect and organize your data into 3 categories. (What are these categories?)

Describe the process used to eliminate organisms from the strike chart. (What do you strike? How many strikes to eliminate?)

Cite the source of the data on your strike chart in parenthetical in-text APA citation format.

Conclusion 05-03-21

(Approximately 1-3 complete sentences) (Due when final paper is due)

Give identity of unknown using proper nomenclature.

Add info regarding your unknown (habitat, size, etc.) with 2 proper APA parenthetical citations.

Discuss any issues & how they were resolved.

*This entire Unknown Project Summary should be written in 3rd person as a proper paper using complete sentences (no bullets) to develop proper paragraphs.

This is just an example. Your submission will have the headings shown here in blue but will not have the questions or suggestions shown here in black and green. Just delete these and replace with your information.

The Unknown Summary will include five components each of which should be typed, double spaced and should use proper sentence/paragraph structure. It should all be written in third person.

This should be an overview of how the project is done in general, and how the process was applied in your specific case. It should give context to the remainder of the paper.

Introduction, Plan, Evidence, Analysis, and Conclusion. • The Introduction and Plan will help with your understanding of the objective

for the Unknown Project. • The Evidence will provide the rationale for which Strike Chart you select. • The Analysis compiles the significant results that will allow you to identify

your organism. • The Conclusion states the identity of your unknown organism.

Due Dates for each component: • Introduction and Plan: at the beginning of the Real Unknowns • Evidence: when you select your Strike Chart • Analysis and Conclusion: with the final draft of paper

Unknown Summary

• Describe the objective of the Unknown Project in 2-3 sentences. • Identify the problem to be considered including relevant

information already known. • What skills and techniques will help to solve the problem?

(These skills may have been learned in this course but may also include life skills.)

• Note: The summary is not cited unless you bring in unique information not mentioned in the rest of the paper. (Only the analysis and conclusion will contain citations.)

Introduction (Due the first week of project)

• Write (in one to two paragraphs) the plan for the steps to be taken to identify an unknown organism. The plan should be broad — any student could use these steps as a guideline to begin identification of their unknown organism. The plan should include:
• How to establish a pure culture? Why? • How to characterize the organism at each step? Why? Consider

characterization of the organism in broth culture, as a colony on an agar plate, and on a slant. (Do not give results.)

• Considering the first page of the Gram-Positive and Gram-Negative flow charts, what would be performed to select a strike chart?

Plan (Due the first week of the project)

• The evidence to select a Strike Chart will use the steps from your plan but will be unique to your unknown organism. Evidence (in 1 paragraph) will include:
• Considering the first page of the Gram-Positive and Gram-Negative flow charts, what did you perform (staining, biochemical testing) to direct you to your strike chart?
• What strike chart did these tests lead to? How?

Evidence (Due upon determination of Strike Chart)

• The analysis to identify your organism should be a brief summary of the necessary tests you performed and how these significant results allowed you to identify your unknown organism. Include (in 1 – 2 paragraphs) the following:
• Briefly summarize how the Descriptive Chart organizes data by type (morphological, cultural, and physiological data).
• Describe the process used to eliminate other possible organisms. • Cite the scientific literature used to compare your results with

published results for each organism. Hint: Each strike chart has a reference for the source of the information given on the chart. Convert to proper APA parenthetical in-text format. *The full APA citation for this reference should also be on your Work Cited Page at the end of your paper.

Analysis (Due in final draft of Unknown Paper)

• The conclusion will state the identification of your unknown organism and any issues that complicated its identification in 1 – 3 sentences.
• Give name of organism using proper nomenclature. • Add info about your organism (habitat, cell size, etc.) with 2 propers

APA parenthetical citations. • Did you have any issues (atypical tests, media availability,

contamination, etc.) that confounded the identification of your organism? How were these issues resolved?

Conclusion (Due in final draft of Unknown Paper)

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